3KN5

Crystal structure of the C-terminal kinase domain of msk1 in complex with AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-07
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	51.561, 91.250, 135.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.240 - 2.400
R-factor	0.211
Rwork	0.208
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qr8
RMSD bond length	0.006
RMSD bond angle	1.104
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.240	2.700
High resolution limit [Å]	2.396	2.400
Rmerge	0.118	0.492
Number of reflections	23504
<I/σ(I)>	16	2.8
Completeness [%]	91.0	85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		PH 7.5, VAPOR DIFFUSION