3KN5
Crystal structure of the C-terminal kinase domain of msk1 in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.561, 91.250, 135.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.240 - 2.400 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qr8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.240 | 2.700 |
| High resolution limit [Å] | 2.396 | 2.400 |
| Rmerge | 0.118 | 0.492 |
| Number of reflections | 23504 | |
| <I/σ(I)> | 16 | 2.8 |
| Completeness [%] | 91.0 | 85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PH 7.5, VAPOR DIFFUSION |
