3KN1
Crystal Structure of Golgi Phosphoprotein 3 N-term Truncation Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97951 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 174.369, 174.369, 174.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zih |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.779 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.133 | 0.586 |
| Number of reflections | 10180 | |
| <I/σ(I)> | 7.8 | 2.4 |
| Completeness [%] | 98.3 | 99.6 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.9 M (NH4)2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
