3KMQ
G62S mutant of foot-and-mouth disease virus RNA-polymerase in complex with a template- primer RNA, tetragonal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.661, 93.661, 121.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.110 |
| R-factor | 0.24144 |
| Rwork | 0.240 |
| R-free | 0.26738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wne |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.841 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | |
| Number of reflections | 59922 | |
| <I/σ(I)> | 14.9 | 3 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% PEG 4000, 0.2M magnesium acetate, 0.1M HEPES pH 7.0, 4% butyrolactone, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
