3KMP

Crystal Structure of SMAD1-MH1/DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	105
Detector technology	CCD
Collection date	2008-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	73.938, 77.490, 83.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.413 - 2.700
R-factor	0.208
Rwork	0.206
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.355
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	13623
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Ammonium tartrate dibasic, 20% PEG 3350, 10% glycerol, 3% 2-propanol (additive), vapor diffusion, hanging drop, temperature 291K