3KML
Circular Permutant of the Tobacco Mosaic Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 220.835, 177.175, 102.801 |
| Unit cell angles | 90.00, 99.55, 90.00 |
Refinement procedure
| Resolution | 49.261 - 3.011 |
| R-factor | 0.24 |
| Rwork | 0.239 |
| R-free | 0.25300 |
| Starting model (for MR) | One ring of 17 monomers generated using PDB ID 1EI7. |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 2009_02_15_2320_3)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 73255 | |
| <I/σ(I)> | 7.5 | 1.6 |
| Completeness [%] | 94.8 | 81.8 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 300 mM ammonium sulfate, 100 mM Nitric Acid, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
