3KM8
Crystal structuore of adenosine deaminase from mus musculus complexed with 9-deazainosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-11 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.337, 93.616, 85.416 |
| Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
| Resolution | 37.480 - 2.000 |
| R-factor | 0.2031 |
| Rwork | 0.202 |
| R-free | 0.22308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1add |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.675 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.820 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.101 | 0.436 |
| Number of reflections | 48057 | |
| <I/σ(I)> | 12.5 | 3 |
| Completeness [%] | 90.8 | 84.7 |
| Redundancy | 9.53 | 6.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% PEG 2000 MME, 150mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
