3KLY

Pentameric formate channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-31
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	100.795, 101.030, 192.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.460 - 2.100
R-factor	0.18
Rwork	0.179
R-free	0.20900
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	102406
<I/σ(I)>	12.43
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2M TRIS, PH 7.5, 28% (W/V) PEG 550MME, 100MM NACL, 20MM SODIUM FORMATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K