3KLL
Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180-maltose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9760 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.165, 65.850, 82.317 |
| Unit cell angles | 73.44, 78.57, 86.34 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3klk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.403 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.747 | 2.100 |
| High resolution limit [Å] | 1.997 | 2.000 |
| Number of reflections | 75992 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 97.5 | 94 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 3350, BIS-TRIS propane buffer, CaCl2, pH 6.0, vapor diffusion, hanging drop, temperature 293K |
