3KL1
Crystal structure of abscisic acid receptor PYL2 at 1.55 A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97943, 0.97960, 0.96438 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.143, 61.143, 184.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.22430 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.667 | |
| Number of reflections | 57490 | |
| <I/σ(I)> | 0.056 | 3.4 |
| Completeness [%] | 97.0 | 5 |
| Redundancy | 10.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 298 | 20mM tris, 30% Peg4k, pH 8.0, EVAPORATION, temperature 298K |
| 1 | EVAPORATION | 8 | 298 | 20mM tris, 30% Peg4k, pH 8.0, EVAPORATION, temperature 298K |
