3KKQ
Crystal structure of M-Ras P40D in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.947, 70.754, 43.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.510 - 1.200 |
| R-factor | 0.17658 |
| Rwork | 0.176 |
| R-free | 0.19222 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x1r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.161 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.110 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.286 |
| Number of reflections | 62215 | |
| <I/σ(I)> | 8.4 | 2.6 |
| Completeness [%] | 98.4 | 97.3 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG-4000, sodium acetate, ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
