3KKP
Crystal structure of M-Ras P40D in complex with GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 33.720, 65.705, 74.077 |
| Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
| Resolution | 27.000 - 1.350 |
| R-factor | 0.18227 |
| Rwork | 0.182 |
| R-free | 0.19514 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.220 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.890 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.045 | 0.268 |
| Number of reflections | 33462 | |
| <I/σ(I)> | 11.1 | 2.4 |
| Completeness [%] | 94.9 | 92.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG-1500, magnesium sulfate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
