3KKN
Crystal structure of H-Ras T35S in complex with GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 34.583, 82.000, 121.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.670 - 2.090 |
| R-factor | 0.19552 |
| Rwork | 0.193 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.670 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.100 |
| Rmerge | 0.088 | 0.282 |
| Number of reflections | 10645 | |
| <I/σ(I)> | 45.4 | 14.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.3 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | MES, Ammonium Sulfate, PEG-MME-5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
