3KKD
Structure of a putative tetr transcriptional regulator (pa3699) from pseudomonas aeruginosa pa01
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.321, 82.879, 46.618 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.20707 |
| Rwork | 0.204 |
| R-free | 0.26182 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.796 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0051) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 | 0.643 |
| Number of reflections | 35089 | |
| <I/σ(I)> | 35.27 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 25% PEG2KMME, CaCl2 0.05 M, 0.1 M Bis-Tris, 0.3 M NDSB 195, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
