3KK0
Crystal structure of partially folded intermediate state of peptidyl-tRNA hydrolase from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.006, 65.597, 68.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.990 - 2.650 |
| R-factor | 0.237 |
| Rwork | 0.223 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kjz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.990 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 6733 | |
| Completeness [%] | 98.1 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20mM Tris-HCl, 1mM EDTA, 50mM NaCl, 5mM 2-mercaptoethanol, 5% (w/v) PEG 400, 10% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
