3KIV

RECOMBINANT KRINGLE IV-10/M66 VARIANT OF HUMAN APOLIPOPROTEIN(A)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	130
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	24.300, 45.670, 63.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 1.800
R-factor	0.182 *
Rwork	0.182
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	0.039
Data reduction software	RIGAKU (R-AXIS)
Data scaling software	R-AXIS ((RIGAKU))
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	2.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.060	0.101 *
Total number of observations	12758 *
Number of reflections	5656
<I/σ(I)>	12.8	4.7
Completeness [%]	81.0	53
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	reservoir	EACA		10-fold molar excess
3	1	reservoir	2-propanol	20 (%)
4	1	reservoir	PEG4000	20 (%)
5	1	reservoir	sodium citrate	0.1 (M)