3KIN

KINESIN (DIMERIC) FROM RATTUS NORVEGICUS

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	72.150, 91.850, 141.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.100
R-factor	0.289
R-free	0.36200
Structure solution method	MIRAS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.000
Rmerge	0.064 *
Total number of observations	113071 *
Number of reflections	19516
<I/σ(I)>	10.7
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	19 *	HANGING DROP METHOD WAS USED. PROTEIN WAS CRYSTALLIZED FROM 20MM PIPES, PH 7.5, 200MM NACL, 2MM DTT, 2 MM NA-EGTA, 0.8 M AS. THE RESERVOIR CONTAINED 1.6 M AS IN THE SAME BUFFER., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	PIPES	20 (mM)
3	1	drop	dithiothreitol	2 (mM)
4	1	drop	Na EGTA	2 (mM)
5	1	drop		200 (mM)
6	1	drop	ammonium sulfate	0.8 (M)
7	1	reservoir	ammonium sulfate	1.6 (M)