3KIK
Sgf11:Sus1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54059 |
| Spacegroup name | P 61 |
| Unit cell lengths | 75.153, 75.153, 197.373 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.860 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2FWB residues 21-90 of chain C |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.517 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.757 |
| Number of reflections | 36176 | |
| <I/σ(I)> | 26.2 | 2.4 |
| Completeness [%] | 98.3 | 95.3 |
| Redundancy | 13.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 3.2 M Na formate. See publication for details, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
