3KIE
Crystal structure of adeno-associated virus serotype 3B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9186 |
Spacegroup name | H 3 |
Unit cell lengths | 257.767, 257.767, 607.037 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 13.926 - 3.000 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lp3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.694 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.070 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.067 | |
Number of reflections | 76529 | |
<I/σ(I)> | 5.2 | 1.92 |
Completeness [%] | 25.4 | 20.4 |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.3 | 298 | 3.2-3.4% PEG 6000, 50mM magnesium chloride, 100mM hepes, pH 7.3, hanging drop, temperature 298K |