3KIC
Crystal structure of adeno-associated virus serotype 3B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9186 |
| Spacegroup name | H 3 |
| Unit cell lengths | 257.704, 257.704, 603.766 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.990 - 2.603 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lp3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.746 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.660 |
| High resolution limit [Å] | 2.600 | 6.410 | 2.600 |
| Rmerge | 0.066 | 0.047 | |
| Number of reflections | 147388 | ||
| <I/σ(I)> | 14.4 | ||
| Completeness [%] | 32.1 | 36.3 | 12.2 |
| Redundancy | 1.2 | 1.2 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.3 | 298 | 3.2-3.4% PEG 6000, 50mM magnesium chloride, 100mM hepes, pH 7.3, hanging drop, temperature 298K |
