3KHZ
Crystal Structure of R350A mutant of Staphylococcus aureus metallopeptidase (Sapep/DapE) in the apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.750, 133.520, 67.710 |
| Unit cell angles | 90.00, 95.59, 90.00 |
Refinement procedure
| Resolution | 49.020 - 2.500 |
| R-factor | 0.21077 |
| Rwork | 0.208 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3khx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.160 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.068 | 0.414 |
| Number of reflections | 39597 | |
| <I/σ(I)> | 20.9 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch method under oil | 6.5 | 293 | 0.2M Ammonium Sulfate, 0.1M MES, 30.0% PEG Mono-methyl-ether 5000, pH 6.5, Microbatch method under oil, temperature 293K |
