3KH5

Crystal Structure of Protein MJ1225 from Methanocaldococcus jannaschii, a putative archaeal homolog of g-AMPK.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Collection date	2009-09-19
Spacegroup name	H 3 2
Unit cell lengths	108.548, 108.548, 143.627
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.231
Rwork	0.228
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PROTEIN MJ0100
RMSD bond length	0.009
RMSD bond angle	1.768
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100	2.100
Number of reflections	19237
<I/σ(I)>	45.2
Completeness [%]	100.0
Redundancy	24.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	291	2.4 M Ammonium sulfate, 2.8 % PEG 400, 100 mM Hepes, 114 mM FOS-choline 8 or 11 mM FOS-choline 10, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K