3KGW

Crystal structure of Putative aminotransferase (AAH25799.1) from MUS MUSCULUS at 1.65 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-18
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	61.360, 112.729, 117.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.683 - 1.650
R-factor	0.171
Rwork	0.169
R-free	0.19800
Structure solution method	MR
Starting model (for MR)	1h0c
RMSD bond length	0.017
RMSD bond angle	1.783
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.683	29.680	1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.079	0.029	0.967
Total number of observations		8200	51619
Number of reflections	98429
<I/σ(I)>	16.6	20.4	0.8
Completeness [%]	100.0	98.5	100
Redundancy	7.1	6.6	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	20.0000% polyethylene glycol 3350, 0.2000M potassium acetate, No Buffer pH 7.8, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K