3KGR
Crystal structure of the human leukocyte-associated Ig-like receptor-1 (LAIR-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0396 |
| Spacegroup name | P 32 |
| Unit cell lengths | 69.395, 69.395, 54.258 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.698 - 1.800 |
| R-factor | 0.124 |
| Rwork | 0.123 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model from Swiss-model based on alignment with PDB entries 2GI7 2d3v 1ugn 2gw5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.279 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.098 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.208 |
| Total number of observations | 12152 | |
| Number of reflections | 26097 | |
| <I/σ(I)> | 20.2 | 3.5 |
| Completeness [%] | 96.3 | 78.4 |
| Redundancy | 5.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 291 | 25-30% PEG-1500, 100mM succinic acid, 100mM sodium dihydrogen phosphate, 100mM glycine, 10mM CoCl2, pH 4.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
