3KFW

Uncharacterized protein Rv0674 from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 42 21 2
Unit cell lengths	85.383, 85.383, 80.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.000 - 2.500
R-factor	0.233
Rwork	0.230
R-free	0.28700
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.789
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.086	0.529
Number of reflections	10757
<I/σ(I)>	37	3.8
Completeness [%]	99.2	98.9
Redundancy	20	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1M Tris HCl pH 8.5, 25% PEG 3350, 0.2M Ammonium Acetate, Vapor diffusion, Sitting drop, temperature 298K