3KFE
Crystal structures of a group II chaperonin from Methanococcus maripaludis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.450, 161.920, 147.370 |
| Unit cell angles | 90.00, 124.12, 90.00 |
Refinement procedure
| Resolution | 54.490 - 3.500 |
| R-factor | 0.2334 |
| Rwork | 0.232 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.490 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 49417 | |
| Completeness [%] | 96.5 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M Li2SO4 and 20 % PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
