3KFB
Crystal structure of a group II chaperonin from Methanococcus maripaludis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 260.694, 162.220, 184.733 |
| Unit cell angles | 90.00, 135.05, 90.00 |
Refinement procedure
| Resolution | 48.840 - 3.200 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.4_162)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.840 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 74731 | |
| Completeness [%] | 100.0 | 83.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 200 mM sodium fluoride and 20% PEG 3,350 , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
