3KEV

X-ray crystal structure of a DCUN1 domain-containing protein from Galdieria sulfuraria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	.97625
Spacegroup name	P 1 21 1
Unit cell lengths	36.743, 52.422, 49.113
Unit cell angles	90.00, 111.17, 90.00

Refinement procedure

Resolution	20.984 - 1.300
R-factor	0.156
Rwork	0.155
R-free	0.17800
Structure solution method	MAD, MR
RMSD bond length	0.018
RMSD bond angle	1.566
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.040	0.388
Number of reflections	42243
<I/σ(I)>	16	2.6
Completeness [%]	98.5	87.2
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	30% PEG 1500, 100 mM sodium acetate pH 5.0, 150 mM MgCl2, 50 mM NaCl, 5 mM HEPES pH 7.0, 0.3 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 293K
2	VAPOR DIFFUSION, SITTING DROP	5	293	27% PEG 1500, 100 mM sodium acetate pH 5.0, 150 mM LiCl2, 50 mM NaCl, 5 mM HEPES pH 7.0, 0.3 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 293K