3KES
Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae in the Hexagonal, P61 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 61 |
| Unit cell lengths | 135.052, 135.052, 56.154 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.520 - 2.100 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.494 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (C) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.920 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 34126 | |
| <I/σ(I)> | 21.2 | 8.2 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 21.7 | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1600mM tri-sodium citrate dehydrate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
