3KEA

Structure function studies of vaccinia virus host-range protein K1 reveal a novel ankyrin repeat interaction surface for K1s function

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2007-11-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793453, 0.9794771
Spacegroup name	P 21 21 2
Unit cell lengths	95.107, 110.263, 86.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.280 - 2.300
R-factor	0.212
Rwork	0.210
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.436
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.280
High resolution limit [Å]	2.300
Number of reflections	37088

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	293	0.2M Natartrate, 20% PEG3350, 0.1M NaAc, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K