3KDS
apo-FtsH crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 190.500, 190.500, 152.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.757 - 2.601 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| Starting model (for MR) | 2ce7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.911 |
| Number of reflections | 50229 | 3602 |
| <I/σ(I)> | 20.59 | 1.9 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 10.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG400, 200mM CaCl2, 100mM Hepes, 200mM glycine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
