3KDQ
Crystal structure of a functionally unknown conserved protein from Corynebacterium diphtheriae.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940, 0.97953 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.676, 132.484, 176.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.461 - 3.000 |
| R-factor | 0.2006 |
| Rwork | 0.197 |
| R-free | 0.26430 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.095 | 0.679 |
| Number of reflections | 18599 | |
| <I/σ(I)> | 29 | 3.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Na-Hepes, 200mM Na Nitrate, 30% PEG400 , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
