3KDN
Crystal structure of Type III Rubisco SP4 mutant complexed with 2-CABP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.474, 246.241, 133.080 |
| Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
| Resolution | 42.860 - 2.090 |
| R-factor | 0.21463 |
| Rwork | 0.213 |
| R-free | 0.25286 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1geh |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.768 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 345208 | |
| <I/σ(I)> | 15.4 | 2.3 |
| Completeness [%] | 96.5 | 70.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M Acetate, 80mM CaCl2, 6% PEG6000, 10% MPD, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
