3KDA
Crystal structure of the CFTR inhibitory factor Cif with the H269A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9464 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.960, 83.640, 88.930 |
| Unit cell angles | 90.00, 100.49, 90.00 |
Refinement procedure
| Resolution | 30.722 - 1.500 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | initial model obtained from SAD with selenomethionine labeled Cif |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.066 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.980 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 192680 | |
| <I/σ(I)> | 22.59 | 5.25 |
| Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 20% PEG 8000, 0.55M calcium chloride, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
