3KCP
Crystal structure of interacting Clostridium thermocellum multimodular components
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 169.977, 58.019, 56.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.490 - 1.940 |
| R-factor | 0.20567 |
| Rwork | 0.206 |
| R-free | 0.23109 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.679 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.490 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.066 | 0.424 |
| Number of reflections | 41456 | |
| <I/σ(I)> | 9.6 | 3.2 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 6.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100 mM MES, 200 mM Ammonium sulfate, 30% w/v PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
