3KCN
The crystal structure of adenylate cyclase from Rhodopirellula baltica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.930, 103.096, 33.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.552 - 2.450 |
| R-factor | 0.2198 |
| Rwork | 0.217 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.498 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.100 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.133 | 0.313 |
| Number of reflections | 11538 | |
| <I/σ(I)> | 15 | 9.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 13.3 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 0.1 M HEPES sodium pH 7.5, 1.4 M sodium citrate tribasic dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 110K |
