3KBQ
The crystal structure of the protein CinA with unknown function from Thermoplasma acidophilum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 143.596, 39.718, 55.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.990 - 2.000 |
| R-factor | 0.20479 |
| Rwork | 0.203 |
| R-free | 0.24130 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.641 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.990 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.114 | 0.773 |
| Number of reflections | 20972 | |
| <I/σ(I)> | 23.5 | 2.64 |
| Completeness [%] | 98.5 | 97.64 |
| Redundancy | 8.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 40% PEG600, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
