3KBP

KIDNEY BEAN PURPLE ACID PHOSPHATASE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-04-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	132.700, 347.300, 128.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.188 *
Rwork	0.189
R-free	0.22100 *
RMSD bond length	0.019
RMSD bond angle	2.600 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000
High resolution limit [Å]	3.000
Rmerge	0.080
Total number of observations	153663 *
Number of reflections	50898
Completeness [%]	85.3	79.5 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	12 (mg/ml)
2	1	drop	PEG10000	4 (%)
3	1	drop	sodium acetate	50 (mM)
4	1	reservoir	PEG10000	15 (%)