3KB3

Crystal structure of abscisic acid-bound PYL2 in complex with HAB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-16
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.319, 67.466, 143.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.520 - 1.950
R-factor	0.217
Rwork	0.214
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.867
Data reduction software	HKL-2000
Phasing software	PHASER (1.3.2)
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.950
Number of reflections	41123
Completeness [%]	86.7
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	PEG 3350, pH 6.5, VAPOR DIFFUSION, temperature 298K