3KAM
Hen Egg White Lysozyme Derivatized with rhenium(I) diaquatricarbonyl cation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-08-21 |
| Detector | OXFORD RUBY CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.709, 78.709, 36.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.990 - 1.590 |
| R-factor | 0.18271 |
| Rwork | 0.181 |
| R-free | 0.21465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lyz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.546 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.990 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.091 | 0.666 |
| Number of reflections | 15125 | |
| <I/σ(I)> | 47.22 | 1.73 |
| Completeness [%] | 99.7 | 96.9 |
| Redundancy | 22.4 | 15.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 0.1 M MES, pH 5.5, 8% NaCl, 50 mg/mL protein, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
