3KAA
Structure of Tim-3 in complex with phosphatidylserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 71.922, 71.922, 263.579 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.934 - 3.002 |
| R-factor | 0.2357 |
| Rwork | 0.235 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oyp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 | 0.339 |
| Number of reflections | 5225 | |
| <I/σ(I)> | 6 | 2.2 |
| Completeness [%] | 94.3 | 90.7 |
| Redundancy | 4.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | paraffin oil | 5.6 | 294 | 22% PEG 4000, 50mM NaF, 5mM CaCl2, 0.1M sodium citrate pH 5.6, paraffin oil, temperature 294K |
