3K9Q
Crystal structure of C151G mutant of Glyceraldehyde 3-phosphate dehydrogenase 1 from Methicillin resistant Staphylococcus aureus (MRSA252) at 2.5 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.933, 105.142, 90.760 |
| Unit cell angles | 90.00, 107.48, 90.00 |
Refinement procedure
| Resolution | 27.240 - 2.500 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3H48 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.651 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.8L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.570 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.089 | 0.286 |
| Total number of observations | 14800 | |
| Number of reflections | 42157 | |
| <I/σ(I)> | 9.9 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.72 | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | 0.1M TRIS-HCl pH8.2, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
