3K9J
Transposase domain of Metnase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.10546 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.158, 45.411, 90.678 |
| Unit cell angles | 90.00, 113.81, 90.00 |
Refinement procedure
| Resolution | 44.585 - 1.903 |
| R-factor | 0.2061 |
| Rwork | 0.205 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.4_4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.060 | 0.033 | 0.425 |
| Number of reflections | 43992 | ||
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 94.2 | 95.5 | 71.1 |
| Redundancy | 3.9 | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | NH4OAc, CaCl2, PEG4000, pH 8.0, vapor diffusion, temperature 293K |
