3K9I

Crystal structure of Putative protein binding protein (NP_241345.1) from Bacillus halodurans at 2.71 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97925,0.94926,0.97939
Spacegroup name	P 61 2 2
Unit cell lengths	73.656, 73.656, 115.838
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.875 - 2.710
R-factor	0.221
Rwork	0.221
R-free	0.23300
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.530
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.875	42.875	2.810
High resolution limit [Å]	2.710	5.800	2.710
Rmerge	0.056	0.028	0.731
Number of reflections	5493	645	530
<I/σ(I)>	25.03	50.7	3.1
Completeness [%]	99.8	99.1	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.07	293	3.5M sodium formate, 0.1M HEPES pH 7.07, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K