3K90
The Abscisic acid receptor PYR1 in complex with Abscisic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.810, 61.280, 72.550 |
| Unit cell angles | 105.65, 102.23, 89.91 |
Refinement procedure
| Resolution | 24.670 - 2.000 |
| R-factor | 0.20209 |
| Rwork | 0.200 |
| R-free | 0.23394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cnw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 50927 | |
| Completeness [%] | 93.3 | 86.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M sodium magnesium acetate, 0.1M sodium cacodylate pH 6.5, 18% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
