3K8V
Crysatl structure of a bacterial cell-surface flagellin N20C20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-29 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.676, 52.619, 64.834 |
| Unit cell angles | 100.32, 109.54, 111.62 |
Refinement procedure
| Resolution | 29.580 - 2.100 |
| R-factor | 0.2151 |
| Rwork | 0.212 |
| R-free | 0.26828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zbi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.055 | 0.163 |
| Number of reflections | 31986 | |
| Completeness [%] | 96.4 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
