3K8V

Crysatl structure of a bacterial cell-surface flagellin N20C20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-29
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	52.676, 52.619, 64.834
Unit cell angles	100.32, 109.54, 111.62

Refinement procedure

Resolution	29.580 - 2.100
R-factor	0.2151
Rwork	0.212
R-free	0.26828
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zbi
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.055	0.163
Number of reflections	31986
Completeness [%]	96.4	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP