3K8P
Structural basis for vesicle tethering by the Dsl1 complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793, 0.9795, 0.9641 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.020, 90.820, 213.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.197 - 2.600 |
| R-factor | 0.2082 |
| Rwork | 0.205 |
| R-free | 0.27080 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 82563 | |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES, pH 7.5, 10% PEG monomethyl ether 5000, 0.1 M MgCl2, 5% dimethyl sulfoxide, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
