3K8N
Crystal structure of E. Coli CCMG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-09-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.696, 67.403, 159.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.080 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kng |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.288 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.100 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 17017 | |
| <I/σ(I)> | 14.2 | 2.4 |
| Completeness [%] | 98.8 | 95.8 |
| Redundancy | 5.56 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | drops contained 1:1 protein:precipitant, where precipitant solution was 30% (w/v) PEG 4000, 0.2M ammonium sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
