3K8M
Crystal structure of SusG with acarbose
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 41 |
| Unit cell lengths | 127.709, 127.709, 127.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| Rwork | 0.201 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k8k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.287 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 71321 | |
| <I/σ(I)> | 20.4 | 2.3 |
| Completeness [%] | 88.9 | |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 22% PEG 4000, 50 mM LiSO4, 100mM HEPES, 10mM acarbose, 0.5mM CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
