3K80
Structure of essential protein from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-01-13 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.278, 52.499, 85.845 |
| Unit cell angles | 90.00, 104.61, 90.00 |
Refinement procedure
| Resolution | 27.801 - 2.400 |
| R-factor | 0.2256 |
| Rwork | 0.223 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EQQ for chain C&D and 1I3V for chain A&B |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.400 | 2.350 |
| Number of reflections | 21647 | |
| <I/σ(I)> | 37 | 2.2 |
| Completeness [%] | 92.6 | 60.9 |
| Redundancy | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG4000, 10% iso-propanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
