3K7Y
Aspartate Aminotransferase of Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.8726 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.838, 100.838, 240.367 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 82.080 - 2.800 |
| R-factor | 0.21806 |
| Rwork | 0.214 |
| R-free | 0.29225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q7w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.080 | 3.080 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.097 | 0.487 |
| Number of reflections | 11597 | |
| <I/σ(I)> | 12.8 | 3.1 |
| Completeness [%] | 97.5 | 99.5 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.0 M Ammonium Sulfate, 0.1 M Bis-Tris/HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
